目的建立经产大鼠乳腺增生病病证结合模型。方法雌性经产未孕SD大鼠隔2 d肌肉注射苯甲酸雌二醇注射液1.0 mg.kg-1,连续10次后次日开始每日肌肉注射黄体酮5.0 mg.kg-1,连续5 d;在造模过程中隔日将其装入束缚笼固定过夜,共15次。在末次注射黄体酮次日测量大鼠乳头直径,进行旷场实验;禁食12 h,速眠新麻醉股动脉取血备检,处死取乳腺备检。结果模型组大鼠较正常组乳头肿胀,旷场实验中格停留时间延长,大鼠穿行格数和站立修饰次数减少,站立修饰时间缩短。模型组大鼠血清泌乳素(PRL)、雌二醇(E2)升高,孕醇(P)降低,乳腺腺泡数量增多,体积增大,腺泡腔扩张,充满嗜碱性分泌物,模型大鼠各项指标3周内未恢复至正常水平。结论激素结合束缚方法成功建立经产大鼠肝郁气滞型乳腺增生病模型。该方法造模结果可靠,符合乳腺增生病临床发病病因,方法简单。 Objective To establish a rat model of mammary gland hyperplasia syndrome. Methods Female non-pregnant SD rats were intramuscularly injected with estradiol benzoate injection 1.0 mg.kg-1 every 10 days. The rats were injected intraperitoneally with 5.0 mg.kg-1 of progesterone d; the cage was fixed overnight in a cage for a total of 15 times during the modeling process. In the last injection of progesterone on the next day to measure the size of rat nipples, open-field experiments; fasting 12 h, fast sleep new anesthetized femoral artery blood test, sacrifice take the breast for examination. Results Compared with the normal group, the model group had more swelling of the nipple, longer stay in the open-field test, fewer passing lines and standing modification, shorter standing modification time. Serum prolactin (PRL) and estradiol (E2) were increased, while gestagen (P) was decreased, the number of mammary gland acinar was increased, the volume was increased, the acinar cavity was dilated and the basophilic secretion was secreted in the model group Rat indicators did not return to normal within 3 weeks. Conclusion Hormone binding and binding method successfully established rat liver qi stagnation model of hyperplasia of mammary glands. The method has reliable results in modeling and is in line with the clinical etiology of breast hyperplasia and the method is simple.