论文部分内容阅读
为了对比观察链球菌弱毒苗使用前后罗非鱼肠道免疫相关细胞超微结构的变化,探讨无乳链球菌弱毒苗对罗非鱼黏膜免疫的影响,试验采用体重为(100±10)g,体长为(15±2)cm的健康罗非鱼共30尾,随机分为对照组和试验组,每组15尾,试验组投喂浓度为1.0×109cfu/m L的口服型无乳链球菌GX035弱毒苗,对照组投喂等量未添加疫苗的饲料,免疫后15 d,对前肠、中肠、后肠的超微结构进行常规透射电镜观察。结果表明:与对照组相比,试验组各肠段杯状细胞内黏液分泌泡明显增多,呈分泌旺盛状态;巨噬细胞、浆细胞、肥大细胞等增多,超微结构有变化,呈现功能活跃状态。说明口服无乳链球菌弱毒疫苗可以提高罗非鱼肠道黏膜免疫。
In order to compare the ultrastructural changes of gut-associated cells in tilapia before and after the use of attenuated streptococcal vaccine, the effects of Streptococcus agalactiae attenuated vaccine on the mucosal immunity of tilapia were studied. The body weight of the tilapia was 100 ± 10 g, Totally 30 healthy tilapia (15 ± 2) cm in length were randomly divided into control group and experimental group with 15 rats in each group. The experimental group was fed oral non-milk chain with the concentration of 1.0 × 109cfu / m L Coccidiosis GX035 attenuated vaccine and control group were fed the same amount of vaccine without vaccine. The ultrastructure of foregut, midgut and hindgut were observed by transmission electron microscope at 15 days after immunization. The results showed that compared with the control group, the mucosal secretions of goblet cells in each group were significantly increased and showed a state of exudative secretion. The number of macrophages, plasma cells, mast cells increased, the ultrastructure changed, and the function was active status. Description of oral Streptococcus agalactiae attenuated vaccine can improve intestinal mucosal immunity of tilapia.