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目的：探讨应用简便、快速及无害化的非核素银染端粒重复序列扩增法（ＴＲＡＰ）检测人细胞端粒酶活性。方法：与核素ＴＲＡＰ相比较，采用非核素银染ＴＲＡＰ检测了２９３细胞，并检测了经ＲＮａｓｅ和加热处理的阴性对照标本和ＱＧＹ７７０１、ＳＭＭＣ－７７２１２株人肝癌细胞。结果：１０个以上的２９３细胞均为阳性，而ＲＮａｓｅ和加热处理的标本均阴性；２株人肝癌细胞均阳性；非核素银染ＴＲＡＰ比核素ＴＲＡＰ快１０ｈ左右。结论：非核素银染的ＴＲＡＰ是特异、敏感及快速的端粒酶活性检测方法，证实２株人肝癌细胞株均有端粒酶活性表达。
OBJECTIVE: To investigate the telomerase activity in human cells using a simple, rapid, and destructive method using non-nuclear silver stained telomere repeat amplification (TRAP). METHODS: 293 cells were stained with non-nuclear silver-stained TRAP compared to radionuclide TRAP, and negative control samples treated with RNase and heat and QGY7701, SMMC-77212 human hepatoma cells were detected. RESULTS: More than 10 293 cells were positive, but RNase and heat-treated specimens were negative; 2 human hepatoma cells were positive; non-nuclear silver-stained TRAP was about 10 h faster than radionuclide TRAP. Conclusion: The non-nuclear silver-stained TRAP is a specific, sensitive and rapid method for detecting telomerase activity, confirming that the expression of telomerase activity in both human hepatoma cell lines is confirmed.