论文部分内容阅读
[目的]研究慈姑多糖的最佳提取工艺及慈姑多糖的抗氧化活性。[方法]考察料液比、提取温度、提取时间、提取次数对慈姑多糖含量的影响,在单因素试验的基础上,采用L9(34)正交试验优化提取工艺参数。通过测定慈姑多糖对DPPH自由基清除率、清除羟自由基活性和还原能力测定等体外抗氧化实验来评价慈姑多糖的体外抗氧化能力。[结果]慈姑多糖的最佳工艺条件为:料液比1∶40(g/ml),提取温度90℃,提取时间4 h,提取次数3次。慈姑多糖的含量为29.32%。1.0 mg/ml慈姑多糖对DPPH自由基清除率为70.62%,对羟基自由基的清除率为35.82%,在还原力的测定中,1.0 mg/ml慈姑多糖在700 nm下吸光度值为0.453 1。[结论]慈姑多糖有较强的抗氧化能力,对体外自由基有较好的清除作用。
[Objective] The research aimed to study the best extraction technology of Sagittaria polysaccharide and the antioxidant activity of Sagittaria polysaccharide. [Method] The effects of material - liquid ratio, extraction temperature, extraction time and extraction times on the polysaccharides content of Radix Astragali were studied. On the basis of single factor experiments, the parameters of L9 (34) orthogonal experiment were optimized. The anti-oxidative ability of Radix Aconiti kaempferi in vitro was evaluated by measuring the DPPH radical scavenging activity, scavenging hydroxyl radical activity and determining the reducing ability of Radix Angelica Sinensis polysaccharides in vitro. [Result] The optimum technological conditions of polysaccharides were: solid - liquid ratio 1:40 (g / ml), extraction temperature 90 ℃, extraction time 4 h and extraction times 3 times. Sagittaria polysaccharide content of 29.32%. The DPPH radical scavenging rate of 1.0 mg / ml Saguar polysaccharide was 70.62% and the scavenging rate of hydroxyl radical was 35.82%. The absorbance value of 1.0 mg / ml Sagittar polysaccharide at 700 nm was 0.453 1 in the determination of reducing power. [Conclusion] Sagittaria polysaccharide has strong antioxidant capacity and good scavenging effect on free radicals in vitro.