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固真方(VRF)由何首乌、肉苁蓉等六味中药组成,具有补肾益精、延缓衰老的作用。能显著提高大鼠成骨样细胞甲状旁腺素(PTH)受体的结合率,增进PTH刺激后细胞内C~(2+)释出的反应性,显著促进细胞增殖有关的原癌基因表达、DNA合成以及骨细胞特征性蛋白——碱性磷酸酶的合成。实验用放射免疫分析法(RIA)研究了VRF提取液对成骨样细胞内环磷酸腺苷(cAMP)基础水平及PTH激发水平的影响。1 材料 大鼠成骨样细胞株ROS17/2.8、cAMP抗体及大鼠PTH(1—34)(美国麻省总医院内分泌室转赠);VRF提取液(水提醇沉提取,1ml相当于15g生药,上海中医药大学中药系提供,使用时用无血清F_(12)培养液
Guzhenfang (VRF) is composed of six Chinese herbal medicines, including Radix Polygoni multiflori and Cistanche deserticola. It has the effects of nourishing the kidney, essence, and anti-aging. It can significantly increase the binding rate of rat parathyroid hormone (PTH) receptors in rat osteoblast-like cells, increase the reactivity of intracellular C~(2+) release after PTH stimulation, and significantly promote the expression of proto-oncogenes related to cell proliferation. , DNA synthesis and synthesis of alkaline phosphatase, a protein characteristic of bone cells. Radioimmunoassay (RIA) was used to study the effect of VRF extract on the basic level of cyclic adenosine monophosphate (cAMP) and PTH stimulation in osteoblast-like cells. 1 Rat osteoblast-like cell line ROS17/2.8, cAMP antibody and rat PTH (1-34) (exchanged from the endocrinology department of the Massachusetts General Hospital of the United States); VRF extract (water extraction and alcohol precipitation, 1 ml equivalent to 15g crude drug , Shanghai University of Traditional Chinese Medicine, Department of Traditional Chinese Medicine, use serum-free F_ (12) medium