论文部分内容阅读
【目的】为菜豆红粉病菌的诊断和综合防治提供理论依据。【方法】按照常规组织分离法和柯赫氏法则从菜豆上分离出致病菌并进行致病性测定。结合形态学特征和ITS序列分析鉴定致病菌,确定其分类地位。采用生长速率法对病原菌碳氮源的利用能力进行测定。【结果】该病菌主要为害菜豆豆荚,在豆荚表面形成圆形或近圆形褐色、凹陷的病斑,且病斑表面后期出现粉红色霉层;病原菌分生孢子倒梨形,单孢,无色,成熟孢子中间有一隔膜,大小(6.23-12.42)μm×(12.07-24.67)μm,经r DNA-ITS序列相似性分析,该病原菌与已报道的粉红单端孢菌Trichothecium roseum(KC816070)相似性达99%以上,结合形态特征,将其鉴定为粉红单端孢菌Trichothecium roseum。该病原菌对不同碳氮源的利用程度有差异,其菌落在麦芽糖培养基上生长速度最快,且在各种碳源培养基上的生长速度均显著高于对照(P<0.05);在不同氮源培养基上,含L-亮氨酸培养基上菌落生长速度最快,含L-精氨酸和尿素上的生长速度显著小于对照(P<0.05)。【结论】研究结果对粉红单端孢菌引起的其他植物病害的研究有一定的参考价值。
【Objective】 The purpose of this study is to provide a theoretical basis for diagnosis and comprehensive prevention and control of bean powdery mildew. 【Method】 Pathogenic bacteria were isolated from kidney beans and pathogenicity was determined according to the method of routine tissue separation and Koch ’s law. Combined with morphological characteristics and ITS sequence analysis identified pathogenic bacteria, to determine its taxonomic status. The growth rate method was used to determine the utilization ability of pathogen carbon and nitrogen sources. 【Result】 The results showed that the pathogen mainly infects peas bean pods and forms a circular or nearly circular brown, concave lesion on the surface of the pod, and the pink mold layer appears on the surface of the lesion. The conidia of the pathogen are pear-shaped, single spore, There was a membrane in the middle of the mature and mature spores with the size of (6.23-12.42) μm × (12.07-24.67) μm, which was similar to the reported Trichothecium roseum (KC816070) The purity of Trichothecium roseum was more than 99%. According to its morphological characteristics, it was identified as Trichothecium roseum. The pathogen showed different degrees of utilization of different carbon and nitrogen sources. The colonies grew fastest on maltose medium and grew faster on all kinds of carbon source medium than on the control (P <0.05) The growth rate of colonies on L-leucine medium was the fastest on nitrogen medium, and the growth rate on L-arginine and urea was significantly less than that on the control (P <0.05). 【Conclusion】 The results of this study have certain reference value for the study of other plant diseases caused by Tricuspidianus.