【摘 要】
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Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purificationof biological substances directly from unclarified feedstock
【机 构】
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Department of Biochemical Engineering
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Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purificationof biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an importantprotein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarifiedhomogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provideda single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and columnwashing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bedmode in the purification of GroEL from cell disruptate.
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