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目的:验证基于血氧水平依赖(BOLD)的多回波梯度自旋回波(MEGSE)序列无创定量测量肾脏氧摄取分数(OEF)等氧代谢指标参数的可行性。方法:15只健康新西兰大白兔入组研究。对每只兔子施行左肾动脉部分结扎手术以造成单侧肾动脉狭窄(RAS)动物模型。行MRI扫描采集3个不同时刻的MEGSE数据,分别为基础状态、RAS术后30min(RAS-30min)和RAS术后60min(RAS-60min),以获得肾脏动脉狭窄造模前后的肾脏OEF值。结果:15只兔子均造模成功。在RAS术前测得的肾脏基础状态下OEF值为(0.36±0.08)(皮质)和(0.39±0.11)(髓质)。RAS术后30min和60min测得的OEF显著增大分别为(0.46±0.14)(皮质,RAS-30min,P<0.05)、(0.51±0.12)(髓质,RAS-30min,P<0.05)、(0.46±0.13)(皮质,RAS-60min,P<0.05)、(0.56±0.09)(髓质,RAS-60min,P<0.05)。结论:本研究验证了MEGSE序列可以无创、定量测量肾缺血状态下的OEF改变,为将来进一步应用于人类肾脏氧摄取分数的测量奠定了基础。
OBJECTIVE: To verify the feasibility of noninvasively quantifying parameters of oxygen metabolism such as kidney Oxygen uptake (OEF) based on the multi-echo gradient echo (MEGSE) sequence of blood oxygen level dependent (BOLD). Methods: Fifteen healthy New Zealand white rabbits were enrolled in this study. A partial ligation of the left renal artery was performed on each rabbit to create a unilateral renal artery stenosis (RAS) animal model. MEGSE data of 3 different time points were acquired by MRI scans, and were respectively basal state, RAS-30min after RAS and 60min (RAS-60min) after RAS to obtain the kidney OEF value before and after renal artery stenosis. Results: All 15 rabbits were successful in modeling. OEF values were (0.36 ± 0.08) (cortex) and (0.39 ± 0.11) (medulla) at baseline renal status measured prior to RAS. OEFs measured at 30 and 60 min after RAS were significantly higher (0.46 ± 0.14 vs. 0.51 ± 0.12, RAS-30 min, P <0.05) (0.46 ± 0.13) (Cortex, RAS-60min, P <0.05), (0.56 ± 0.09) (Medulla, RAS-60min, P <0.05). CONCLUSIONS: This study demonstrates that MEGSE sequence can be used as a noninvasive and quantitative measure of OEF changes in renal ischemic state, which lays the foundation for the further measurement of oxygen uptake in human kidney in the future.