论文部分内容阅读
目的：探讨躯体性及心理性应激状态下大鼠胃粘膜ＮＯＳ活性变化及其与胃粘膜损伤的关系。方法：将９６ 只雄性ＳＤ大鼠随机分为３组：对照组，规则光（Ｒ）组及不规则光（Ｉ）组，采用高压恒流脉冲刺激器制备躯体性和心理性应激模型。分光光度法检测胃粘膜组织的ＮＯＳ活性和Ｎｉｌｓ Ｌａｍ ｂｅｃｈｔ法测定胃粘膜损伤指数。结果：正常情况下ＮＯＳ活性平稳，应激后大鼠胃粘膜ＮＯＳ活性均明显升高，Ｉ组升高程度更明显；与对照组比较，Ｒ，Ｉ组胃粘膜损伤指数均较高，且Ｉ组较Ｒ组变化显著（Ｐ＜ ０．０５）。结论：心理性应激程度愈高，胃粘膜损伤愈严重，胃粘膜损伤程度及ＮＯＳ活性变化与心理应激程度关系密切
Objective: To investigate the changes of gastric mucosal NOS activity and its relationship with gastric mucosal injury in somatic and psychological stress rats. Methods: Ninety-six male Sprague-Dawley rats were randomly divided into three groups: control group, regular light (R) group and irregular light group (I). Hypobaric constant current stimulator was used to prepare the somatic and psychological stress models. The activity of NOS in gastric mucosa was detected by spectrophotometry and the gastric mucosal injury index was measured by Nils Lam becht method. Results: The activity of NOS was stable under normal conditions, and the gastric mucosal NOS activity was significantly increased in the stress group and the degree of increase was more obvious in the I group. Compared with the control group, gastric mucosal injury index of R and I group were higher, and I Compared with R group, the changes were significant (P <0.05). Conclusion: The higher the degree of psychological stress, the more serious gastric mucosal injury, the degree of gastric mucosal injury and changes in NOS activity and psychological stress are closely related