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用离子选择电极电位法研究了银和铅与人白蛋白（ＨＳＡ）的配合平衡。明确证实银和铅都与ＨＳＡ形成了单核配合物ＭＡ和ＭＡ２．Ａｇ－ＨＳＡ配合物的稳定性积β（１１）＝４．４×１０７，β（１２）＝１．０×１０（１２）；Ｐｂ－ＨＳＡ配合物的β（１１）＝３．１×１０５、β（１２）＝２．０×１０９。根据ＨＳＡ与人白蛋白羊抗血清（ＧＡＨＡ）的免疫反应，用银和铅分别标记ＨＳＡ时，测定ＧＡＨＡ（效价１：４０）的线性范围是０．２～２．０μＬ和０．４～２．８μＬ；而用银和铅分别标记ＧＡＨＡ时，测定ＨＳＡ的线性范围是０～２．９×１０（－８）ｍｏｌ／Ｌ和０～７．３×１０（－７）ｍｏｌ／Ｌ。
The balance of silver and lead with human albumin (HSA) was studied by ion-selective electrode potentiometry. It was clearly confirmed that both silver and lead formed mononuclear complexes MA and MA2 with HSA. The stability of Ag-HSA complex is shown in the following table: β (11) = 4.4 × 107, β (12) = 1.0 × 10 , Β (12) = 2.0 × 109. According to the immune response of HSA and human albumin antiserum (GAHA), the linear range of GAHA (titer 1:40) was 0.2 ~ 2.0μL and 0.4 ~ The linear range of HSA was 0 ~ 2.9 × 10 (-8) mol / L and 0 ~ 7.3 × 10 (-7) mol / L when GAHA was labeled with silver and lead, respectively.