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目的建立灵敏测定生物样品中皮克级枸橼酸芬太尼的方法。方法使用流动注射-化学发光法、鲁米诺-溶解氧体系检测芬太尼,蠕动泵流速2.0mL/min,鲁米诺与NaOH混合后被六通阀定量注入系统直至形成稳定基线,同时将样品推进送入混合管。碱性混合液被送入化学发光流通池产生化学发光,光电倍增管和光度计即检测到发光信号强度。结果在1.0～700pg/mL线性范围内,化学发光强度与芬太尼浓度呈线性关系,且发光强度随浓度增大呈增敏趋势,检出限低至0.3pg/mL。相对标准偏差在3.0%以内。分析过程可在30s内完成,采样频率120次/h。回收率98.9%～103.1%。结论该方法已经成功应用于检测人体血清中的枸橼酸芬太尼。
Objective To establish a sensitive method for the determination of Pico-grade fentanyl citrate in biological samples. Methods Flow-injection chemiluminescence was used to detect fentanyl with luminol-dissolved oxygen system. The flow rate of peristaltic pump was 2.0 mL / min. After the mixture of luminol and NaOH was injected into the system quantitatively through a six-way valve, a steady baseline The sample is pushed into the mixing tube. The alkaline mixture is sent to the chemiluminescence cell for chemiluminescence, and the photomultiplier tubes and photometers detect luminescence signal intensities. The results showed that there was a linear relationship between the chemiluminescence intensity and the concentration of fentanyl in the linear range of 1.0 ~ 700pg / mL, and the luminescence intensity tended to increase with the increase of the concentration, the detection limit was as low as 0.3pg / mL. The relative standard deviation is within 3.0%. Analysis process can be completed within 30s, the sampling frequency of 120 times / h. Recovery rate of 98.9% ~ 103.1%. Conclusion This method has been successfully applied to the detection of human serum fentanyl citrate.