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目的:采用超高压液相色谱-二级管阵列检测器-四级杆-飞行时间质谱(UHPLC-DAD-Q-TOF-MS/MS)联用技术,在负离子模式下开展血人参水煎煮提取物中化学成分的一级及二级质谱研究,分析血人参的水溶性化学成分。方法:液相色谱条件为Synergi Hydro-RP色谱柱(2.0 mm×100 mm,2.5μm),0.1%甲酸乙腈-0.1%甲酸水溶液梯度洗脱。质谱条件为Bruker Daltonics micro TOF-Q质谱仪,电喷雾离子源,负离子模式,毛细管电压2 800 V,二级质谱碰撞能量11~25 e V;源内碰撞诱导裂解(CID)及其二级质谱条件:源内CID能量25~35 e V,碰撞能量12~17 e V。结果:共推测出15个化合物的化学结构,分别为没食子酸、原儿茶酸-3-O-葡萄糖苷、原儿茶酸、原儿茶醛、丁香酸葡萄糖苷、龙胆酸、儿茶素、表儿茶素、原花青素B类同分异构体4个、棕儿茶素A类同分异构体3个。结论:除化合物表儿茶素外,其他14个化合物均为该植物首次报道。研究结果为血人参及其制剂的开发和质量标准制定提供了依据。
OBJECTIVE: To study the effect of decoction of Radix Panacis Quinquefolii by using UHPLC-DAD-Q-TOF-MS / MS combined with ultra-high pressure liquid chromatography-diode array detector- The chemical constituents of the extract of the first and second-level mass spectrometry, analysis of the water-soluble chemical composition of ginseng. Methods: The liquid chromatographic conditions were Synergi Hydro-RP column (2.0 mm × 100 mm, 2.5 μm) with a gradient of 0.1% formic acid in acetonitrile and 0.1% formic acid. Mass spectrometry conditions were Bruker Daltonics micro TOF-Q mass spectrometer, electrospray ionization source, negative ion mode, capillary voltage 2 800 V, and secondary mass spectrometry collision energy 11-25 eV; collision-induced lysis (CID) : Source CID energy 25-35 eV, collision energy 12-17 eV. Results: The chemical structures of 15 compounds were speculated as gallic acid, protocatechuic acid-3-O-glucoside, protocatechuic acid, protocatechuic aldehyde, syringic acid glucoside, gentisic acid, Epicatechin, proanthocyanidin class B isomers 4, catechin class A isomers 3. Conclusion: All the 14 compounds except epicatechin were reported for the first time. The results provided the basis for the development and quality standardization of Radix Ginseng and its preparations.