应用同一切片,顺序Fos-ABC-DABni和5-HT-PAP-DAB法在电针及痛组大鼠中进行双免疫细胞化学染色法,观察大鼠中脑中缝背核(dr)和延髓头端腹侧区5-HT神经元和Fos免疫阳性胞核的共存研究。观察表明,针刺和痛组动物均可在dr、中缝大核(rm)和巨细胞旁网状外侧核(PGCL)部位观察到Fos和5-HT神经元的共存。但针刺动物在延髓头端腹侧区的共存数较多,远远超过Fos表达数的半数以上。 Double immunocytochemical staining was performed on the same slice in order of Fos-ABC-DABni and 5-HT-PAP-DAB in EA and pain-relieving rats. The dorsal raphe (DR) Coexistence of 5-HT neurons and Fos immunopositive nuclei in the ventral part of the ventral horn. The observation showed that the co-existence of Fos and 5-HT neurons was observed in both the acupoints and painful animals at dr, mesenchymal nucleus (rm), and giant cytoplastic reticular nucleus (PGCL). However, the numbers of coexistence of acupuncture animals in the rostral ventrolateral region were far more than half of those in Fos expression.