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研究了诺卡氏菌在不同影响因素条件下,在黄铁矿和闪锌矿表面的选择性吸附。结果表明:培养120 h后诺卡氏菌达到最大生长量;诺卡氏菌在黄铁矿和闪锌矿表面发生明显的选择性吸附,在黄铁矿表面的最大吸附率可达到96.99%,而在闪锌矿表面的吸附率大都在20%以下;诺卡氏菌在两种矿物表面于20 min内即可达到吸附平衡;pH值是影响诺卡氏菌在两种矿物表面发生选择性吸附的关键因素,pH在4~10之间时,选择性吸附现象明显;矿浆浓度超过6 g/L时有利于发生选择性吸附;细胞悬浊液浓度、搅拌速度和温度对吸附效果影响不大,均可产生选择性吸附。扫描电镜检测结果表明,诺卡氏菌细胞表面的菌丝是重要的吸附部位;红外光谱分析结果表明,诺卡氏菌表面含有—OH、—NH2、C O、P O、S O等活性官能团,它们在吸附过程中起重要作用。
The selective adsorption of Nocardia on the surface of pyrite and sphalerite under different influence factors was studied. The results showed that the maximum growth rate of Nocardia reached to 120 h after culture. Nocardia showed obvious selective adsorption on the surface of pyrite and sphalerite. The maximum adsorption rate on the surface of pyrite could reach 96.99% While adsorption rates on the surface of sphalerite are mostly below 20%. Nocardia can reach adsorption equilibrium within 20 min on both mineral surfaces; pH value affects the selectivity of Nocardia on the two mineral surfaces Adsorption of the key factors, the pH of 4 to 10, the phenomenon of selective adsorption was obvious; pulp concentration of more than 6 g / L is conducive to selective adsorption; cell suspension concentration, stirring speed and temperature on the adsorption effect Large, can produce selective adsorption. Scanning electron microscopy results showed that mycelium on the surface of Nocardia cells was an important adsorption site. Infrared spectroscopy analysis showed that the surface of Nocardia bacteria contained active functional groups such as -OH, -NH2, CO, PO, SO, Adsorption plays an important role.