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本文应用光镜,计算机与显微图像形态定量分析软件相结合的方法,观察到人大肠癌培养细胞经Vit K_3不同剂量(2.5、5、10和20μg/ml)作用后,细胞核DNA指数(DI)明显下降。从DI(倍体)分布直方图中,观察到Vit K_3使癌细胞的DNA主峰由DI=3转向1.5,核的倍体由高异倍体向低异倍体转化〔1〕,表明Vit K_3的抗肿瘤作用与抑制DNA合成和减轻肿瘤恶性度有关。本文提供了一种快速、简便、高精度定量测定DNA含量(IOD)的先进方法和手段。可望对肿瘤诊断、预后估计有一定的参考意义,亦可用于对细胞倍体性改变与肿瘤恶性度关系的分析。
In this paper, light microscopy, computer and microscopic image quantitative analysis software were combined to observe the nuclear DNA index (DI) of human colon cancer cells after different doses of Vit K_3 (2.5, 5, 10, and 20 μg/ml). ) Obvious drop. From the DI (ploidy) distribution histogram, it was observed that Vit K_3 shifted the main DNA peak of cancer cells from DI=3 to 1.5, and the nuclear ploidy was transformed from high to low heteroploid [1], indicating that Vit K_3 The anti-tumor effect is related to inhibition of DNA synthesis and reduction of tumor malignancy. This article provides a quick, easy, and accurate method for the quantitative determination of DNA content (IOD). It is expected to have a certain reference value for tumor diagnosis and prognosis estimation. It can also be used for the analysis of the relationship between cellular ploidy changes and tumor malignancy.