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目的探讨脂联素对肿瘤坏死因子α(TNF-α)诱导大鼠主动脉血管平滑肌细胞血管细胞黏附分子1(VCAM-1)和细胞间黏附分子1(ICAM-1)的表达及其意义。方法复苏培养大鼠血管平滑肌细胞,通过逆转录聚合酶链式反应(RT-PCR)和免疫蛋白印迹法(Western blot)观察脂联素对TNF-α诱导的血管平滑肌细胞VCAM-1、ICAM-1蛋白及mRNA的影响。结果TNFα-(10 ng/ml)刺激后血管平滑肌细胞VCAM-1和ICAM-1表达明显增强(P<0.01);脂联素对其则呈浓度依赖性抑制作用。结论脂联素可抑制TNF-α诱导的血管平滑肌细胞VCAM-1和ICAM-1的表达,这可能对减轻血管壁的炎症反应、延缓动脉粥样硬化的发生、发展起一定作用。
Objective To investigate the expression and significance of adiponectin on the expression of vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in rat aortic vascular smooth muscle cells induced by tumor necrosis factor α (TNF- α). Methods Rat vascular smooth muscle cells (VSMCs) were resuscitated and cultured. TNF-α-induced VCAM-1 and ICAM-1 mRNA expressions were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. 1 protein and mRNA effects. Results The expressions of VCAM-1 and ICAM-1 in VSMCs stimulated by TNFα- (10 ng / ml) were significantly increased (P <0.01). Adiponectin inhibited the expression of VCAM-1 and ICAM-1 in a concentration-dependent manner. Conclusion Adiponectin can inhibit the expression of VCAM-1 and ICAM-1 induced by TNF-α in vascular smooth muscle cells, which may play an important role in alleviating the inflammatory reaction of vascular wall and delaying the occurrence and development of atherosclerosis.