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作者采用改良钩体外腹提取法,提取五株钩体外膜均获成功。电镜观察发现五株钩体外膜超微结构层数存在明显差别。豚鼠保护力试验证实赖型017株外膜对同型强毒株攻击具有明显的保护作用。SDS-PAGE和免疫印迹分析表明017株外膜蛋白具有良好的抗原性。017株和601株外膜蛋白电泳图谱及兔抗血清交叉识别图谱虽非常相似,但存在明显差别。
The authors adopted improved hook body abdomen extraction method, extraction of five hook body outer membrane were successful. Electron microscopy showed that there were significant differences in the ultrastructure of the outer membrane of five hooks. Guinea pig protection test confirmed Lai Lai 017 outer membrane of the same type of virulent strain has a significant protective effect. SDS-PAGE and Western blot analysis showed that 017 outer membrane protein has good antigenicity. There was a significant difference between the electrophoresis patterns of 017 and 601 outer membrane proteins and the rabbit antiserum cross-recognition patterns.