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目的了解达州市食源性疾病主动监测沙门菌流行情况、菌型分布、耐药情况,为预防控制食源性疾病发生和临床治疗提供科学依据。方法由哨点医院采集临床就医腹泻患者粪便或肛拭子标本,按照GB/4789.4进行分离鉴定和血清分型;采用WHO推荐的改良K-B纸片法,2014年采用MIC法对分离的沙门菌进行12种抗生素药物敏感试验。结果42株沙门菌共来自4个群14种血清型,其中B群最多47.62%;血清型以鼠伤寒沙门菌最多28.57%、其次为布利丹沙门菌19.05%、肠炎沙门菌11.90%。对磺胺73.81%、氨苄西林55.88%、四环素52.38%、链霉素41.18%和萘啶酸30.95%耐药,对环丙沙星、头孢他啶较敏感;有36株菌(85.71%)具有耐药性,耐3种以上抗生素的有24株菌(57.14%)。结论达州市哨点医院监测沙门菌血清型别复杂多样,且各种血清型别都存在不同程度耐药,加强对沙门菌的监测监控,及时发现新的血清型和耐药特性,为有效防治由沙门菌引发的食品安全事故和临床用药提供可靠依据。
Objective To understand the prevalence, distribution and drug resistance of Salmonella in foodborne diseases in Dazhou City and provide a scientific basis for prevention and control of foodborne diseases and clinical treatment. Methods Sentinel hospital collected clinical diarrhea patients with fecal or anal swab specimens, in accordance with GB / 4789.4 for identification and serological typing; using the WHO recommended modified KB paper method, in 2014 using the MIC method for isolated Salmonella 12 kinds of antibiotic drug sensitivity test. Results A total of 42 strains of Salmonella were found in 14 serogroups of 4 groups, of which 47.62% were in group B, 29.52% were serotype Salmonella typhimurium, 19.05% were Salmonella bourgeon, and 11.90% were Salmonella enteritidis. Resistant to sulfamethazine 73.81%, ampicillin 55.88%, tetracycline 52.38%, streptomycin 41.18% and nalidixic acid 30.95%, sensitive to ciprofloxacin and ceftazidime 36 strains (85.71%) were resistant 24 strains (57.14%) resistant to more than 3 antibiotics. Conclusion The serotypes of Salmonella in sentinel Hospitals in Dazhou are complex and diverse, and all kinds of serotypes have different degrees of resistance. The surveillance and monitoring of Salmonella is strengthened, and new serotypes and drug resistance characteristics are found in time. Salmonella caused by food safety incidents and clinical medication to provide a reliable basis.