本工作通过用碳二亚胺将β内啡肽与甲状腺球蛋白相偶联,并以此免疫家免,获得了高滴度(1:16000),高亲和力(最小检出量为5 fmol/管,IC_(50)为260 fmol/管,亲和常数为6.35×10~9L/mol)和较好特异性的抗血清,并建立了稳定、可靠的放射免疫测定。用此技术测定大鼠垂体及各脑区的β内啡肽免疫活性物质,结果与文献一致。 In this work, high titers (1: 16000) and high affinity (minimum detectable amount of 5 fmol / L) were obtained by coupling beta endorphin with thyroglobulin using carbodiimide, Tube with IC50 of 260 fmol / tube and affinity constant of 6.35 × 10 ~ 9 L / mol) and better specific antisera. A stable and reliable radioimmunoassay was established. This technique was used to determine the β-endorphin immunoreactive substance in the pituitary and brain regions of rats. The results were consistent with the literature.