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目的 建立并优化室间隔缺损潜在生物标记物细胞间黏附分子1(ICAM-1)MRM-MS 测定方法.方法 借助UniProt 数据库和辅助软件Skyline 建立ICAM-1 多重反应监测质谱定量(MRM-MS)方法,进一步筛选其各肽段及母子离子对,并通过改变MS 的碰撞能量CE 值和去簇电压DP 值进一步优化其MRM 质谱扫描方法.结果 从 ICAM-1 的11 个肽段88 对离子对中优选出3 个肽段9 对离子对作为ICAM-1 的定量依据.采用优化后的MRMMS进行检测,10 个血清样本中ICAM-1 蛋白含量为(318.40 ± 97.20)ng/mL,与经酶联免疫法测定的结果(330.30 ± 130.24)ng/mL 相比差异无统计学意义(P ＞ 0.05).结论 借助UniProt 数据库和辅助软件Skyline,经过多个循环可以建立并优化ICAM-1 蛋白的MRM-MS 测定方法.“,”Objective To establish and optimize a multiple reaction monitoring-mass spectrometry (MRM-MS) method for detection of intercellular adhesion molecule 1 (ICAM-1), which is a potential biomarker for ventricular septal defect. Methods By means of UniProt database and the Skyline software we established a MRM-MS method to detect ICAM-1; then we screened the peptides and the ion pair of ICAM-1 and changed the collision energy value and declustering potential voltage to optimize the MRM-MS method. Results Three peptides and 9 ion pairs were selected from 11 peptides and 88 ion pairs of ICAM-1 as the basis of quantitative determination. Detected with the optimized MRM-MS, the content of ICAM-1 protein in 10 serum samples was 318.40 ± 97.20 ng/ml, which was not significantly different from the result (330.30 ± 130.24 ng/ml) measured with enzyme-linked immunoassay (P ＞ 0.05). Conclusion By means of UniProt database and the Skyline software and several procedures, we established and optimized a MRM-MS method for the detection of ICAM-1.