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目的观察抗辐灵活性成分黄芪总苷、赤芍总苷和丹参酮对微波辐射致PC12细胞氧化应激损伤的保护作用。方法采用神经生长因子(nerve growth factor,NGF)诱导的PC12细胞为模型,分别给予1、3和9μg/ml黄芪总苷、赤芍总苷、丹参酮,作用48 h后经30 m W/cm2微波辐射6 min,于辐射后即刻采用倒置相差显微镜观察PC12细胞形态变化,四甲基偶氮唑盐(methylthiazolyl tetrazolium,MTT)比色法测定细胞生存率,活性氧探针二氯二氢荧光素二乙酸酯(DCFH-DA)法测定细胞内活性氧(reactive oxygen species,ROS)水平,硫代巴比妥酸(thiobarbituric acid,TBA)法测定细胞脂质过氧化代谢产物丙二醛(malonyldialdehyde,MDA)含量。结果 3种活性成分作用后经微波辐射各组PC12细胞形态未见明显差异。辐射后6 h,辐射组PC12细胞较对照组(假辐射组)生存率明显降低(P<0.01);与辐射组相比,黄芪总苷(3μg/ml)给药组PC12细胞生存率明显提高(P<0.01)。辐射后0和48 h,辐射组PC12细胞ROS、MDA水平明显升高(P<0.01),与辐射组相比,各药物预处理组PC12细胞ROS、MDA水平均显著降低(P<0.05)。结论抗辐灵3种活性成分能改善微波辐射对PC12细胞的损伤,其保护作用可能是通过清除氧自由基和减轻氧化应激损伤来实现的。
Objective To observe the protective effects of Radix Astragali, Astragalosides, total paeoniflorin and tanshinone on the oxidative stress injury induced by microwave in PC12 cells. Methods PC12 cells induced by nerve growth factor (NGF) were used as models, and the total astragalosides, total glucosides and tanshinone were respectively administered with 1, 3 and 9 μg / ml, and the cells were exposed to 30 mW / The morphological changes of PC12 cells were observed by inverted phase contrast microscope immediately after irradiation. Cell viability was measured by methylthiazolyl tetrazolium (MTT) colorimetric assay. The activity of reactive oxygen species probe dichlorodifluoro fluorescein di The level of reactive oxygen species (ROS) in cells was measured by DCFH-DA method. The malondialdehyde (MDA), a metabolite of lipid peroxidation, was detected by thiobarbituric acid (TBA) MDA) content. Results There was no significant difference in PC12 cell morphology between the three groups after microwave irradiation. The survival rate of PC12 cells in radiation group was significantly lower than that in control group (sham radiation group) at 6 h after radiation (P <0.01). Compared with radiation group, the survival rate of PC12 cells in astragaloside group (3μg / ml) (P <0.01). The levels of ROS and MDA in PC12 cells in radiation group were significantly increased at 0 and 48 hours after radiation (P <0.01). Compared with radiation group, the levels of ROS and MDA in PC12 cells in each group were significantly decreased (P <0.05). Conclusion Three active ingredients against Radix Erythrinae can improve the damage of PC12 cells under microwave radiation. The protective effect may be through the scavenging of oxygen free radicals and the reduction of oxidative stress injury.