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目的探讨特应质婴儿对常见呼吸道病毒感染的易感性及病毒感染后细胞因子IL-12、IL-27等的表达变化。方法对116例反复喘息的婴幼儿用AllergyScreen变应原检测系统进行屋尘螨粉尘螨、豚草、猫毛皮屑、狗毛皮屑、蟑螂、真菌、葎草、梧桐、鸡蛋、牛奶、鱼、虾、蟹、牛肉、羊肉、腰果、花生、黄豆、芒果、小麦等20种常见吸入性变应原和食入性变应原检测,以sIgE>0.35 U.L-1作为阳性诊断标准,只要有一个阳性即为特应质,否则为非特应质。同时取其鼻咽分泌物用反转录(RT)-PCR方法进行呼吸道合胞病毒、鼻病毒、流感病毒、副流感病毒、人偏肺病毒、冠状病毒(博卡病毒)、肠病毒检测,根据病毒和变应原检测结果将研究对象分为特应质病毒阳性组和非特应质病毒阳性组,用ELISA法测定2组患儿血清IL-12、IL-27水平。结果 116例反复喘息患儿中病毒感染阳性77例,阳性率为66.4%;变应原筛查阳性65例,阳性率为56.0%。特应质病毒阳性组病毒感染阳性率为75.4%,非特应质病毒阳性组病毒感染阳性率为54.9%,二组病毒感染阳性率比较差异有统计学意义(χ2=5.37,P<0.05),二组病毒感染种类比较差异无统计学意义(χ2=0.012 7,P>0.05)。病毒感染时,特应质病毒阳性组患儿血清IL-12、IL-27水平均明显高于非特应质病毒阳性组(t=2.579、2.573,Pa<0.01)。结论呼吸道病毒感染是诱发婴幼儿喘息的主要原因,特应质患儿对呼吸道病毒感染存在易感性。
Objective To investigate the susceptibility of infants with common respiratory tract infection and the changes of cytokines such as IL-12 and IL-27 after special virus infection. Methods One hundred and sixty-six infants with repeated wheezing were treated with AllergyScreen allergen detection system for house dust mite, house dust mite, ragweed, cat dander, dog fur dander, cockroach, fungus, ragweed, paulownia, egg, milk, , 20 kinds of common inhalation allergens and ingestion allergens such as crab, beef, mutton, cashew nut, peanut, soybean, mango and wheat, with sIgE> 0.35 UL-1 as positive diagnostic criteria. For the ad hoc nature, otherwise non-adatom. At the same time take nasopharyngeal secretions by reverse transcription (RT) -PC method for respiratory syncytial virus, rhinovirus, influenza virus, parainfluenza virus, human metapneumovirus, coronavirus (Boka), enterovirus detection, According to the detection results of virus and allergen, the subjects were divided into atypical virus positive group and non-atypical virus positive group. The levels of IL-12 and IL-27 in the two groups were measured by ELISA. Results Of the 116 patients with recurrent wheezing, 77 were positive for viral infection, the positive rate was 66.4%. Allergen screening was positive in 65 cases, the positive rate was 56.0%. The positive rate of virus infection in the positive group was 75.4%. The positive rate of virus infection in the positive group was 54.9%. The positive rate of virus infection in the two groups was statistically significant (χ2 = 5.37, P <0.05) The two groups of virus infection was no significant difference (χ2 = 0.012 7, P> 0.05). Serum levels of IL-12 and IL-27 in patients with positive TSV were significantly higher than those in non-TSV-positive patients (t = 2.579, 2.573, Pa <0.01). Conclusions Respiratory virus infection is the main cause of wheezing in infants and young children, especially children with respiratory tract virus infection susceptibility.