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目的:观察经前舒颗粒对经前期综合征(PMS)肝气郁证模型大鼠下丘脑不同核团雌激素受体(ER)α,ERβ蛋白分布及表达的影响,研究经前舒颗粒治疗该病症的中枢作用机制。方法:将30只雌性SD大鼠随机分为正常对照组、模型组、模型给药组,每组10只。采用慢性束缚应激造模法复制PMS肝气郁证大鼠模型。造模同时,模型给药组按10 g.kg-1剂量ig给药。造模结束后,通过免疫组织化学技术ABC法检测各组大鼠雌激素受体α,β在下丘脑中的定位分布并分析其积分吸光度(IA);HPLC检测各组大鼠下丘脑中5-羟色按(5-HT)含量。结果:3组大鼠雌激素受体ERα,ERβ主要分布在下丘脑的腹内侧区(VMH)核团,分布区域无差异。模型组VMH区ERα,ERβIA分别为163 605±27 284和147 318±24 924与正常组IA(130 156±32 579,105 430±28 641)相比显著升高(P<0.05,P<0.01);给药组ERα,ERβIA为122 020±21 287和77 482±31112,与模型组相比显著降低(P<0.01,P<0.01)。模型组大鼠下丘脑5-HT含量(454.51±65.18)μg.L-1与正常组(144.47±42.20)μg.L-1相比显著升高(P<0.01),给药组(234.11±14.38)μg.L-1与模型组相比5-HT含量显著下降(P<0.01)。结论:下丘脑VMH区ERα,ERβ蛋白表达升高可能是PMS肝气郁证发病的中枢机制之一。经前舒颗粒可有效改善这种状态,提示下丘脑ERα,ERβ可能是该药治疗PMS肝气郁证的部分作用靶点。
Objective: To observe the effect of Jingqianshu granule on the protein distribution and expression of estrogen receptor (ER) α and ERβ in different nuclei of hypothalamus in model rats with PMS, The central mechanism of action. Methods: Thirty female SD rats were randomly divided into normal control group, model group and model group, with 10 rats in each group. Chronic restraint stress model was used to duplicate rat model of PMS liver qi stagnation syndrome. At the same time, the model group was given ig at a dose of 10 g.kg-1. After modeling, the immunohistochemical ABC method was used to detect the distribution of estrogen receptor alpha and beta in the hypothalamus and the integral absorbance (IA) of each group was detected. The content of 5- Color according to (5-HT) content. Results: The estrogen receptors ERα and ERβ in the three groups mainly distributed in the ventral medial region (VMH) of the hypothalamus, with no difference in distribution. The ERα and ERβIA in model group were significantly higher (P <0.05, P <0.01) compared with IA group (130 156 ± 32 579 and 105 430 ± 28 641) in 163 605 ± 27 284 and 147 318 ± 24 924, respectively. The ERα and ERβIA in the treatment group were 122 020 ± 21 287 and 77 482 ± 31112, which were significantly lower than those in the model group (P <0.01, P <0.01). The content of 5-HT in hypothalamus in model group (454.51 ± 65.18) μg.L-1 was significantly higher than that in normal group (144.47 ± 42.20) μg.L-1 (P <0.01) 14.38) μg.L-1, compared with the model group, the content of 5-HT was significantly decreased (P <0.01). Conclusion: The increased expression of ERα and ERβ in hypothalamus VMH may be one of the central mechanisms of PMS. Jingqishu granules can effectively improve this state, suggesting that hypothalamic ERα, ERβ may be part of the drug treatment of PMS liver qi stagnation.