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目的探讨花色苷对CCl_4导致肝纤维化模型小鼠的改善作用,并初步探讨其抗纤维化的作用机制。方法将30只Balb/c小鼠随机分为空白对照组、CCl_4组和CCl_4+花色苷组。空白对照组给予生理盐水灌胃和玉米油腹腔注射,CCl_4组给予生理盐水灌胃和溶于玉米油的10%CCl_4腹腔注射,花色苷组给予10μg/g的花色苷灌胃和溶于玉米油的10%CCl_4腹腔注射。结合常规HE、苦味酸-天狼猩红染色评价肝组织炎症及纤维化程度,Western blot和q-PCR检测肝脏α-平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(COL1A1)以及转化生长因子-β1(TGF-β1)的表达水平,并通过激光共聚焦检测肝星状细胞自噬水平。结果花色苷干预组与CCl_4组相比纤维化指标检测均有降低且差异具有统计学意义(P<0.05),肝星状细胞的活化与自噬水平受到抑制。结论花色苷能够改善CCl_4所致肝纤维化水平,可能与降低肝星状细胞的自噬水平有关。
Objective To investigate the effect of anthocyanins on CCl_4-induced hepatic fibrosis in mice and to explore its anti-fibrosis mechanism. Methods Thirty Balb / c mice were randomly divided into blank control group, CCl 4 group and CCl 4 + anthocyanin group. The blank control group was given saline and corn oil intraperitoneal injection, CCl_4 group was given saline and intraperitoneal injection of 10% CCl_4 dissolved in corn oil, anthocyanin group given 10μg / g of anthocyanin gavage and dissolved in corn oil 10% CCl_4 intraperitoneal injection. The hepatic inflammation and fibrosis were evaluated by routine HE and picric-Sirius red staining. The expressions of α-SMA, COL1A1, and transforming growth Factor-β1 (TGF-β1), and detect the autophagy of hepatic stellate cells by laser scanning confocal microscope. Results Compared with CCl 4 group, the changes of fibrosis indexes in anthocyanin intervention group were significantly lower than those in CCl 4 group (P <0.05), and the activation and autophagy of hepatic stellate cells were inhibited. Conclusion Anthocyanins can improve the level of hepatic fibrosis induced by CCl_4, which may be related to the reduction of autophagy in hepatic stellate cells.