桧柏扦插繁殖过程中,愈伤组织老化使插穗长时间处于不死也不生根的状态(长达1年)。为了解决这个问题,我们进行了促使桧柏插穗老化愈伤组织生根的研究。一、材料和方法(一)材料当年老化愈伤组织取自扦插的木质化插穗,隔年老化愈伤组织取自前1年扦插的嫩枝插穗,采条母株均为3年生植株。(二)方法1.插穗的老化愈伤组织切割取插穗增大的老化愈伤组织进行4种切割处理: (1)切愈:在愈伤组织正中切成“+” Cypress cypress cutting propagation, callus aging cuttings in a long time not dead or not rooted state (up to 1 year). In order to solve this problem, we conducted a study that led to the rooting of aged calli in Juniperus. I. Materials and Methods (I) Materials In the same year, the aged calli were taken from the cuttings of lignified cuttings. The aged calli were taken from the cuttings cuttings of the previous year and the mother trees were all three-year-old plants. (B) Method 1. Cutting callus cut old cut callus take aging cuttings for 4 kinds of cutting: (1) cut more: in the middle of the calluses cut into “+”