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我们于1991年6～8月对我省部分人群进行了莱姆病血清流行病学调查。现报告如下。调查对象及方法在我省北部、中北部和南部选12个县,从中选择了2414人,按照统一的调查表格由调查者询问并填写各项,无菌采各调查对象静脉血或耳血,分离血清置-20℃冻存。调查采用间接免疫荧光试验,抗原片(B_(31)株)由中国预防医学科学院流行病学微生物学研究所提供。羊抗人IgG荧光抗体(冻干)由卫生部兰州生物制品研究所生产,批号91001。判定标准:每视野有50%螺旋体染上荧光,螺旋形态清晰可辨为阳性。血清效价1:64为可疑,1:128及以上为阳性。梅毒TRUST试验:TRUST试剂盒由中国药品生
From June to August 1991, we conducted a serological epidemiological investigation of Lyme disease in some of our province. The report is as follows. Subjects and methods were selected in 12 counties in northern, central and southern part of our province, and 2414 people were selected. According to a unified questionnaire, the investigators asked for and filled in various items, Separation of serum frozen at -20 ℃. The indirect immunofluorescence assay was used in the investigation. Antigen fragment (strain B_ (31)) was provided by Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine. Goat anti-human IgG fluorescent antibody (lyophilized) produced by the Ministry of Health Lanzhou Institute of Biological Products, lot number 91001. Criteria: 50% of spirochetes per field were stained with fluorescence and the spiral morphology was clearly identifiable as positive. Serum titer 1:64 is suspicious, 1: 128 and above are positive. Syphilis TRUST test: TRUST kit by the Chinese medicine students