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以改进的方法培养人脐带静脉血管内皮细胞(HEC),应用Ⅷ因子相关抗原的免疫组化方法进行鉴定,光镜、电镜观察培养细胞的形态变化,并对培养上清液前列环素(PGI_2)及细胞膜ATP酶进行了动态测定。结果表明:原代培养的HEC在一定时间内,其形态与代谢无明显变化。
Human umbilical vein endothelial cells (HECs) were cultured with improved methods and identified by Ⅷ factor - related antigen immunohistochemistry. Morphological changes of cultured cells were observed under light microscope and electron microscope. The cultured supernatant of prostacyclin (PGI_2) ) And cell membrane ATPase were measured. The results showed that there was no significant change in the morphology and metabolism of primary cultured HECs in a certain period of time.