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原发性肝癌是我国南方常见肿瘤疾患,病情凶险,一经诊断确实,多数患者生存期难得超过半年。我们于1975年8月土法上马,开始从脾脏、扁桃体等淋巴组织中提取转移因于(简称TF),配合中草药在一些肝癌患者中使用。为了探索新的免疫疗法,1976年以来,又将人肝癌细胞作为瘤苗,免疫绵羊,然后取绵羊的脾脏、淋巴结用热酚法提取免疫核糖核酸(简称IRNA),对肝癌患者进行试验性治疗,一年来,有3例原发性肝癌患者,经用中草药、肝炎恢复期脾脏提取的转移因子(简称TFH·S)及免疫核糖核酸治疗后获得较明显的疗效,现报告如下: 一、材料与方法转移因子和免疫核糖核酸制作简介: 1.转移因子的提取(超滤法)。在冷冻条件下取肝炎康复患者的脾,去包膜,加适量水,用组织捣碎机匀浆直至无完整细胞为止,然后用80%冷酒精沉淀组织蛋白,调节pH至5.4左右,冰箱过夜,离心,取上清液冷冻干燥,再溶于适量水,在加压3公斤条件下通过特制醋酸纤维膜,取滤液过6号除菌漏斗,
Primary liver cancer is a common tumor in southern China. It is a dangerous condition. Once diagnosed, most patients have a survival period of more than six months. We started indigenous methods in August 1975 and began to extract and transfer from the spleen, tonsils and other lymphoid tissue (abbreviated as TF), and used Chinese herbs in some liver cancer patients. In order to explore new immunotherapy, since 1976, human hepatoma cells have been used as tumor vaccines to immunize sheep, and then the immune system ribonucleic acid (IRNA) has been extracted from the spleen and lymph nodes of sheep using thermal phenol method to conduct experimental treatment of liver cancer patients. In the past year, 3 cases of primary liver cancer patients have obtained more obvious curative effect after being treated with Chinese herbal medicine and the transfer factor extracted from the spleen of hepatitis (abbreviated as TFH·S) and immunoribonucleic acid. The report is as follows: 1. Materials An Introduction to Method Transfer Factor and Immunization RNA Production: 1. Extraction of Transfer Factor (ultrafiltration). Recover the spleen of patients with hepatitis recovered under freezing conditions, remove the envelope, add appropriate amount of water, homogenize with tissue masher until there is no whole cell, then use 80% cold alcohol to precipitate tissue protein, adjust the pH to about 5.4, and the refrigerator overnight. , Centrifuge, take the supernatant liquid to freeze-dry, dissolve in the appropriate amount of water, pass the special cellulose acetate membrane under the pressure of 3 kg, and take the filtrate through the No. 6 sterilization funnel.