鉴于人类裸淋巴细胞迄今未见特异性表面标志,故用去除B和T细胞的负选择法可加以分离。大多数学者采用两步法分离技术,即用尼龙丝吸附或抗Ig柱层析法去除B细胞,继用花环形成试验,借密度梯度离心去除T细胞,这种方法麻烦费时。本文介绍一种简单的一步法双花环分离技术。 Given that so far no specific surface markers have been found in human naked lymphocytes, the negative selection method for removing B and T cells can be used for isolation. Most scholars use two-step separation technique, that is, using nylon filament adsorption or anti-Ig column chromatography to remove B cells, followed by the formation of rosette test, density gradient centrifugation to remove T cells, this method cumbersome and time-consuming. This article describes a simple one-step double-garland separation technique.