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目的通过观察孕期母鼠体内给予尼古丁对其子代肾脏血管紧张素Ⅱ——Ⅰ型受体(AT1R)、Ⅱ型受体(AT2R)的基因及蛋白表达的影响,为研究胎儿肾素血管紧张素系统(RAS)发育及相关胎源性疾病提供理论根据。方法 SD孕鼠随机分为正常组和尼古丁组,分别给予相应处理,于出生14d、5个月处死新生鼠及子代鼠,称量体质量和肾脏质量,计算肾脏指数,测定肾组织AT1R和AT2R mRNA和蛋白表达。结果与正常组相比,尼古丁组新生鼠体质量、肾脏质量及成年子代鼠的肾脏质量及肾脏的AT2R mRNA和蛋白水平表达均明显减少(均P<0.05),AT1R基因、蛋白表达水平无明显差异(P>0.05),但AT1R/AT2R mRNA比值明显升高(P<0.05),而成年子代鼠的体质量无明显差异(P>0.05)。结论孕产期母鼠体内给予尼古丁可致胎鼠出生低体质量,但对成年后体质量没有影响,表现出明显的出生“追赶现象”;孕产期母鼠体内给予尼古丁可影响子代肾脏正常生长及AT2R的表达,从而影响子代肾脏RAS发育及功能的发挥。
Objective To observe the effects of nicotine on the expression of AT1R and AT2R in kidneys of pregnant rats during pregnancy to investigate the effects of nicotine on the expression of fetal renal angiotensin Ⅱ (RAS) development and related fetal diseases provide theoretical basis. Methods Pregnant SD rats were randomly divided into normal group and nicotine group. The neonatal rats and offspring mice were sacrificed 14 days and 5 months after birth. Body weight and kidney mass were measured. The renal index was calculated. The levels of AT1R and AT2R mRNA and protein expression. Results Compared with the normal control group, the body weight and kidney mass of neonatal rats in nicotine group and the kidney mass and the expression of AT2R mRNA and protein in kidney were significantly decreased (all P <0.05). The expression of AT1R gene and protein (P> 0.05), but the ratio of AT1R / AT2R mRNA was significantly increased (P <0.05), while there was no significant difference in body weight among adult offspring rats (P> 0.05). Conclusions Nicotine in pregnant mothers could lead to low birth weight of fetus but no effect on body weight in adulthood, showing obvious “catch-up phenomenon” during pregnancy; nicotine influential in pregnant mothers On behalf of the normal growth of kidney and AT2R expression, thus affecting the offspring kidney RAS development and function of play.