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目的了解广东省女性吸毒者中艾滋病病毒1型(HIV1)亚型的流行规律及其与国际参考株的同源性。方法应用套式聚合酶链反应对20例广东省感染了HIV1的女性吸毒者淋巴细胞富集液的核酸样品进行扩增,并使用ABI377型测序仪对扩增产物测序后,对其env基因C～V段的核酸序列进行比较分析。结果20份血样中,9份为07BC重组亚型,与国际标准株CN97C54A最近,基因离散率为233%±1318,组内离散率为3307%±1483;6份为AE重组亚型,与国际标准株01AETH90CM240最近,基因离散率为8607%±0917,组内离散率为6934%±4560;4份为08BC重组亚型,与国际标准株97CNGX9F最近,基因离散为2708%±1593,组内离散率为3655%±1197;1份为C亚型,与国际标准株95IN21068最近,基因距离为568%。结论广东省女性吸毒者中既存在主要在广东省、广西壮族自治区吸毒人群中流行的08BC重组亚型,又存在主要在西北地区吸毒人群中流行的07BC重组亚型,也存在主要在性途径感染人群中流行的AE重组亚型,提示在女性吸毒者中流行的亚型趋于复杂,女性吸毒者作为HIV从吸毒人群流向一般人群的桥梁作用正在显现,应尽快采取有效措施,防止HIV的进一步蔓延。
Objective To understand the prevalence of HIV1 subtype among female drug users in Guangdong and its homology with the international reference strains. Methods Twenty cases of HIV-infected female HIV-infected lymphocyte-enriched nucleic acid samples from Guangdong Province were amplified by nested polymerase chain reaction (PCR-RFLP). The amplified products were sequenced using ABI377 sequencer. The env gene C ~ V segment of the nucleic acid sequence comparative analysis. Results Among the 20 blood samples, 9 were of the 07BC recombinant subtype. The most recent gene divergence rate was 233% ± 1318 compared with that of the international standard strain CN97C54A. The in-group discrete rate was 3307% ± 1483. The standard strain 01AETH90CM240 recently had a gene dispersal rate of 8607% ± 0917 and an intra-group dispersion rate of 6934% ± 4560; 4 copies of the 08BC recombinant subtype, and the recent international standard strain 97CNGX9F had a gene dispersion of 2708% ± 1593, The rate was 3655% ± 1197; one was subtype C, and the closest to the international standard strain 95IN21068, the gene distance was 568%. Conclusions There are both 08BC subtypes prevailing among drug users in Guangdong Province and Guangxi Zhuang Autonomous Region and 07BC subtypes that are predominant among drug addicts in the northwestern region in Guangdong Province, The prevalence of AE subtypes in the population suggests that the subtypes prevailing in female drug users tend to be complex and that the role of female drug users as a bridge from the drug users to the general population is emerging and effective measures should be taken as soon as possible to prevent HIV from further spread.