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为探索辐射诱导(Egr-1)靶向基因治疗与α粒子照射联合作用对靶区周围肿瘤及正常细胞的影响,将经辐射诱导腺病毒(Ad-ET)处理后的辐照与未受辐照细胞通过共培养体系进行培养,观察受体肿瘤细胞A549和正常细胞MRC-5的生存率和凋亡情况。结果显示,辐射诱导腺病毒Ad-ET联合剂量为0.5 Gy的粒子照射可以对肿瘤细胞A549产生显著的协同抗肿瘤作用,且辐射联合处理组中受体肿瘤细胞的存活率比单独病毒处理组的下降6%,凋亡率显著上升4.9%,而正常细胞中没有此现象发生。结果说明Ad-ET与α粒子照射联合可显著抑制旁区肿瘤细胞生长并引起凋亡,而对正常细胞几乎无影响。证明了辐射诱导的靶向TRAIL基因治疗与辐射联合,可通过特异性增强对靶区周围肿瘤细胞的杀伤进一步提高放射治疗疗效。
In order to explore the effect of radiation-induced (Egr-1) targeted gene therapy and α-particle irradiation on the tumor and normal cells around the target area, the irradiated and non-irradiated According to the co-culture system, the cells were cultured to observe the survival rate and apoptosis of the recipient tumor cell A549 and the normal cell MRC-5. The results showed that radiation-induced adenovirus Ad-ET combined dose of 0.5 Gy of particles can have a significant synergistic anti-tumor effect on A549 tumor cells, and radiation combined treatment group, the survival rate of the recipient tumor cells than the virus-treated group alone A 6% decrease, and a significant increase of 4.9% in apoptosis rate, which is not observed in normal cells. The results show that the combination of Ad-ET and α-particle irradiation can significantly inhibit the growth of adjacent tumor cells and cause apoptosis, while almost no effect on normal cells. It is demonstrated that radiation-induced targeting of TRAIL gene therapy in combination with radiation can further enhance the efficacy of radiation therapy by specifically killing the tumor cells surrounding the target area.