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微生物灌浆封堵混凝土裂缝具有渗透性好、生成物性质稳定和结合性好等优点。微生物诱导碳酸钙沉积物的产量成为影响裂缝修复效果的重要因素。文章通过对比试验,研究了营养液浓度、钙源溶液种类、钙源浓度和尿素加入方式对微生物诱导碳酸钙产量的影响规律。结果表明:尿素溶液浓度为3 mol/L,钙源溶液采用硝酸钙浓度为2 mol/L,或氯化钙浓度为3 mol/L,单位体积溶液内碳酸钙沉淀物产量分别为0.11和0.12 g/mL;菌液中提前60 min加入尿素,10 min后碳酸钙的产量是不提前加入尿素产量的5.6倍,从而有效地提高了碳酸钙的早期产量,有利于实现裂缝的快速封堵;采用优化组的灌浆比未优化组的灌浆次数明显减少。
Microbial grouting plugging concrete cracks with good permeability, the stability of the product properties and good combination of advantages. Microbial induced calcium carbonate sediment yield has become an important factor affecting the fracture repair effect. Through comparative experiments, the article studied the effects of nutrient solution concentration, type of calcium source solution, calcium source concentration and urea adding method on the microbial-induced calcium carbonate production. The results showed that the concentration of urea solution was 3 mol / L, the concentration of calcium source solution was 2 mol / L or the concentration of calcium chloride was 3 mol / L, the yield of calcium carbonate precipitate per unit volume was 0.11 and 0.12 g / mL. The urea solution was added 60 min earlier in the bacterial solution. After 10 min, the output of calcium carbonate was 5.6 times of that of urea added in advance, which effectively increased the early yield of calcium carbonate and facilitated the rapid plugging of the fracture. The grouting frequency of the optimized group was significantly reduced compared with the un optimized group.