HIV通过感染CD4~+辅助T淋巴细胞导致免疫系统的全面崩溃。CD4是一位于细胞表面并能介导细胞与HIV包膜糖蛋白gp120之间相互作用的蛋白质分子。在体内,HIV可产生新的变异株而逃脱抗体的中和作用,但此变异株却仍可作用于CD4以持续感染的循环。因此,能用可溶性CD4蛋白抑制HIV吸附于靶细胞,从而阻断HIV的感染。作者根据免疫球蛋白表达系统制备了两种杂交基因。HT4-Y1编码CD4的类免疫球蛋白区及相继的三个功能区——即整个细胞外部分。另一杂交基因HT4-X6仅编码类免疫球蛋白区及第二功能区。C端蛋白部分均 HIV causes complete breakdown of the immune system by infecting CD4 ~ + helper T lymphocytes. CD4 is a protein molecule that resides on the cell surface and mediates the cell’s interaction with the HIV envelope glycoprotein gp120. In the body, HIV can produce new variants to escape the neutralization of antibodies, but the mutant still acts on CD4 to sustain the cycle of infection. Thus, soluble CD4 protein can be used to inhibit the adsorption of HIV to target cells, thereby blocking HIV infection. The authors prepared two hybrids based on the immunoglobulin expression system. HT4-Y1 encodes CD4-like immunoglobulin domains and three subsequent functional domains - the entire extracellular portion. The other hybrid gene, HT4-X6, encodes only the immunoglobulin-like region and the second functional region. C-terminal protein parts are