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已知人类的表皮郎格罕细胞(LC)在皮肤的免疫应答中起着重要的作用.为此,作者将结核菌素阳性个体和(或)患有复发性唇部单纯疱疹患者,以及对镍有接触变应性皮炎患者的一定浓度的自体外周血T细胞与表皮细胞(含3～6%的LC)、或血液单核细胞(系巨噬细胞)在结核菌素纯蛋白衍化物(PPD)、单纯疱疹病毒(HSV)抗原和不同浓度的硫酸镍刺激下,于37℃含5%CO_2的湿环境下共同孵育培养5天,用氚胸腺嘧啶核苷掺入测定抗原特异的T细胞应答.结果发现外周血单核细胞和表皮LC对诱导PPD的T细胞应答具有同样强的能力.在患复发性唇部单纯疱疹的患者中,在诱导HSV
It is known that the human epithelial Langerhans cell (LC) plays an important role in the skin’s immune response.To this end, the authors identified tuberculin-positive individuals and / or patients with recurrent lip herpes as well as Nickel has certain concentrations of autologous peripheral blood T cells and epidermal cells (containing 3 to 6% of LC) in contact with allergic dermatitis or blood mononuclear cells (macrophages) in the presence of tuberculin pure protein derivative PPD), herpes simplex virus (HSV) antigen and different concentrations of nickel sulfate, cultured in a humid environment of 5% CO 2 at 37 ° C for 5 days, mixed with tritiated thymidine to measure antigen-specific T cells Response. As a result, it was found that peripheral blood mononuclear cells and epidermal LC have the same strong ability to induce T cell responses to PPD. In patients with recurrent herpes simplex, in the induction of HSV