论文部分内容阅读
目的研究人参皂苷Rb2对金黄色葡萄球菌的体外抗感染活性,为人参皂苷在抗感染类疾病中的应用提供理论依据。方法采用胞外侵入试验和纤粘连蛋白黏附试验研究Rb2对金黄色葡萄球菌感染鼠肺上皮细胞的作用,并用RT-PCR方法检测对金黄色葡萄球菌相关基因表达的影响。结果人参皂苷Rb2不能抑制金黄色葡萄球菌的生长,但是在浓度>0.1μmol/L时能够通过调节纤粘连蛋白相关基因agr、sarA和fnbA的表达降低金黄色葡萄球菌表面纤粘连蛋白对宿主细胞的黏附能力,从而降低对鼠肺上皮细胞的侵染。结论人参皂苷Rb2的体外抗感染活性具有独立于机体免疫系统外的途径,主要通过调节金黄色葡萄球菌表面纤粘连蛋白相关基因的表达而降低对宿主的侵染。
Objective To study the anti-infective activity of ginsenoside Rb2 against Staphylococcus aureus in vitro and to provide a theoretical basis for the application of ginsenosides in anti-infective diseases. Methods The effect of Rb2 on the lung epithelial cells infected by Staphylococcus aureus was studied by extracellular invasion assay and fibronectin adhesion assay. The effects of Rb2 on the expression of related genes of Staphylococcus aureus were detected by RT-PCR. Results Ginsenoside Rb2 could not inhibit the growth of Staphylococcus aureus but could reduce the effect of Staphylococcus aureus surface fibronectin on host cells by regulating the expression of fibronectin related genes agr, sarA and fnbA at concentrations> 0.1 μmol / L Adhesion, thereby reducing the infection of mouse lung epithelial cells. CONCLUSION Ginsenoside Rb2 has anti-infective activity in vitro independently of the immune system of the host and reduces host infection mainly by regulating the expression of fibronectin related genes on the surface of S. aureus.