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目的:检测RPR持续阳性经治梅毒患者外周血淋巴细胞亚群,探讨其传染性与细胞免疫的相关性。方法:应用流式细胞仪检测RPR持续阳性梅毒患者中38例IgM阳性和32例IgM阴性的患者外周血T细胞亚群及NK细胞,并以30例健康人作为对照组。结果:IgM阳性组外周血CD3、CD4和CD8细胞均高于对照组(P<0.05),而NK细胞低于对照组(P<0.001);IgM阴性组外周血CD3、CD4细胞低于对照组(P<0.05),而CD8细胞及NK细胞均高于对照组(P<0.05);两组之间外周血淋巴细胞亚群的检测结果相比,IgM阳性组CD3、CD4细胞高于IgM阴性组(P<0.001),NK细胞低于IgM阴性组(P<0.001),而CD8细胞相比差异无显著性(P>0.05)。结论:RPR持续阳性梅毒患者存在明显的细胞免疫不平衡和免疫抑制;NK细胞显著降低可能是RPR持续阳性且具传染性的主要原因。
Objective: To detect peripheral blood lymphocyte subsets of patients with RPR persistent positive syphilis, and to explore the correlation between infectious and cellular immunity. Methods: T-cell subsets and NK cells in peripheral blood of 38 patients with IgM positive and 32 IgM negative patients with RPR persistent positive syphilis were detected by flow cytometry. Thirty healthy controls were used as control group. Results: The levels of CD3, CD4 and CD8 in peripheral blood in IgM positive group were significantly higher than those in control group (P <0.05), while NK cells were lower in control group (P <0.001). CD3 and CD4 in peripheral blood of IgM negative group were lower than those in control group (P <0.05), while the levels of CD8 and NK cells in control group were higher than those in control group (P <0.05). Compared with the results of peripheral blood lymphocyte subsets in both groups, CD3 and CD4 in IgM positive group were higher than that in IgM (P <0.001). NK cells were lower than those in IgM negative group (P <0.001), but there was no significant difference between CD8 cells (P> 0.05). CONCLUSIONS: Patients with RPR persistent positive syphilis have obvious cellular immune imbalance and immunosuppression. The significant decrease of NK cells may be the main reason that RPR is persistent and contagious.