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目的 探讨甲醛对ICR小鼠学习记忆能力的影响及机制.方法 以ICR小鼠为实验对象,实验设对照组和甲醛高、中、低剂量组(200、150、1 00 mg/kg);连续给予甲醛8周,采用新物体辨别及Morris水迷宫实验评价小鼠学习记忆能力,酶联免疫实验检测神经营养因子的表达.结果 与对照组比较,中、高剂量甲醛组小鼠新物体辨别实验优先指数[分别为(0.49±0.11)、(0.48±0.08)]明显下降,水迷宫实验第3天逃避潜伏期[分别为(56.51±8.01)、(53.46±10.64)s]明显延长(P＜0.01),小鼠海马组织中脑源性神经营养因子(BDNF)表达水平[分别为(121.19±3.07)、(101.87±2.95)pg/mL]与神经生长因子(NGF)表达水平[分别为(54.44±2.64)、(33.93±4.06)pg/mL]明显降低.结论 甲醛可引起ICR小鼠学习记忆障碍,其机制可能与降低小鼠脑内神经营养因子水平有关.“,”Objective To investigate the influence of formaldehyde on learning and memory ability in ICR mice.Methods The ICR mice were assigned into a control group and high,moderate,and low (200,150,100 mg/kg) formaldehyde groups with consecutive administration of formaldehyde for 8 weeks.The learning and memory abilities of the mice were evaluated with novel object recognition test and Morris water maze.The expression of neurotrophic factor was detected with enzyme-linked immunosorbent assay.Results Compared with those of the control group,the priority index (0.49 ± 0.11,0.48 ± 0.08) was significantly decreased in high and moderate formaldehyde groups;the avoidance latency of the third-day Morris water maze test (56.51 ± 8.01,53.46 ± 10.64 seconds) was significantly prolonged (both P ＜ 0.01).The levels of brain derived neurotrophic factor (BDNF) (121.19 ± 3.07,101.87 ± 2.95 pg/mL) and nerve growth factor (NGF) (54.44 ± 2.64,33.93 ± 4.06 pg/mL) in hippocampus of high and moderate formaldehyde groups were significantly lower than those in the control group.Conclusion The administration of formaldehyde could result in learning and memory impairments in ICR mice and the effect may be related to the decrease of neurotrophic factors in the brain of the mice.