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[目的]探讨诱导型一氧化氮合酶(iNOS)在喉癌组织中的表达与血管内皮生长因子-C(VEGF-C)、淋巴管密度(LVD)、淋巴结转移和预后的相关性。[方法]以45例经病理确诊的喉癌组织为实验组,20例喉良性病变组织为对照组,采用免疫组织化学检测iNOS和VEGF-C的表达,采用5′-核苷酸酶染色(5′-Nase)计数LVD,并结合临床病理特征和生存资料进行相关分析。[结果]喉癌组织中iNOS和VEGF-C的阳性表达率分别为71.1%和37.8%,较对照组显著增加(P<0.05),在喉癌组织中iNOS表达与瘤内和瘤周LVD、淋巴结转移、TNM临床分期、淋巴管浸润有关(P<0.05)。iNOS和VEGF-C表达正相关(r=0.396,P<0.05),iNOS阳性表达者生存率显著低于阴性表达者(P<0.05)。[结论]喉癌组织中iNOS过表达可能通过上调VEGF-C表达促进喉癌淋巴管生成和淋巴结转移,检测iNOS可成为判断喉癌预后新的生物学指标。
[Objective] To investigate the correlation between the expression of inducible nitric oxide synthase (iNOS) and the expression of vascular endothelial growth factor-C (VEGF-C), lymphatic vessel density (LVD), lymph node metastasis and prognosis in laryngeal carcinoma. [Method] Totally 45 cases of laryngeal carcinoma confirmed by pathology were used as experimental group and 20 cases of laryngeal benign lesion as control group. The expressions of iNOS and VEGF-C were detected by immunohistochemical staining. 5’-Nase) count LVD, combined with clinical and pathological features and survival data for correlation analysis. [Results] The positive rates of iNOS and VEGF-C in laryngeal carcinoma were 71.1% and 37.8%, respectively, which were significantly higher than those in control group (P <0.05). The expression of iNOS in laryngeal carcinoma was significantly correlated with the LVD, Lymph node metastasis, TNM clinical stage and lymphatic invasion (P <0.05). iNOS and VEGF-C were positively correlated (r = 0.396, P <0.05). The survival rate of iNOS positive patients was significantly lower than that of negative ones (P <0.05). [Conclusion] Overexpression of iNOS in laryngeal carcinoma may promote lymphangiogenesis and lymph node metastasis of laryngeal carcinoma by up-regulating the expression of VEGF-C. Detecting iNOS may be a new biological index to judge the prognosis of laryngeal carcinoma.