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目的:以Con A诱导小鼠免疫性肝损伤模型,研究狭基线纹香茶菜对免疫肝损伤小鼠ICAM-1mRNA和蛋白表达的影响,探讨其抗免疫肝损伤的作用机理。方法:小鼠尾静脉注射ConA 25mg/kg制备免疫肝损伤模型,灌胃给予狭基线纹香茶菜水提物7d后,不同时间点取血检测血清ALT、AST水平,取肝组织,提取总RNA反转录聚合酶链式反应(RT-PCR)对肝脏ICAM-1mRNA进行半定量测定,免疫组化法检测肝组织ICAM-1蛋白的表达。结果:与正常对照组相比,模型组小鼠血清AST、ALT水平明显高于正常组,狭基线纹香茶菜水提物9.2g/kg可明显降低肝损伤小鼠血清ALT、AST水平。肝组织4h ICAM-1 mRNA和蛋白表达显著增强,至8h仍保持较高的表达。阳性药PDTC和狭基线纹香茶菜水提物大剂量组ICAM-1mRNA和蛋白表达显著低于模型组。结论:ConA损伤后,血清ALT、AST水平升高,肝组织中ICAM-1mRNA和蛋白表达明显增强,狭基线纹香茶菜组可通过降低损伤肝组织ICAM-1mRNA和蛋白的表达,而对免疫肝损伤小鼠起保护作用,这可能是其护肝降酶作用机理之一。
OBJECTIVE: To study the effect of Conch-induced Acute Hepatic Injury in mice on the expression of ICAM-1 mRNA and protein in mice with immune-induced liver injury and the mechanism of its anti-immune liver injury. Methods: The model of immunocompromised liver injury was induced by injection of ConA 25mg / kg into the caudal vein of mice. Serum ALT and AST were measured by gavage at different time points. The liver ICAM-1 mRNA was semi-quantitatively determined by RT-PCR and the expression of ICAM-1 protein was detected by immunohistochemistry. Results: Compared with the normal control group, serum AST and ALT levels in the model group were significantly higher than those in the normal control group. The aqueous extract of the basilar line Xiangxiangcao 9.2g / kg significantly reduced the levels of serum ALT and AST in the liver injury mice. The mRNA and protein expression of ICAM-1 in liver tissue increased significantly at 4h, and remained high at 8h. The expression of ICAM-1mRNA and protein in positive drug PDTC and narrow baseline Glycyrrhiza uralensis water extract high dose group were significantly lower than those in model group. CONCLUSIONS: The levels of serum ALT and AST are increased after ConA injury, and the expression of ICAM-1mRNA and protein in liver tissues are significantly increased. The narrow base line Xiangchacao group can reduce the expression of ICAM-1mRNA and protein in liver tissues, Liver injury mice play a protective role, which may be one of the mechanisms of its role in liver enzymes.