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目的:研究维生素D受体(VDR)基因启动子FokⅠ多态性与良性前列腺增生(BPH)合并组织学前列腺炎的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对79例BPH合并前列腺炎患者(炎症组)及81例单纯BPH患者(对照组)的外周血进行FokⅠ多态性检测,分析两组间基因型和等位基因的频率分布。结果:炎症组基因型分布FF:27%(21/79),Ff:30%(24/79),ff:43%(34/79);对照组FF:33%(27/81),Ff:36%(29/81),ff:31%(25/81),其中ff基因型分布在两组中差异有统计学意义(P<0.05)。炎症组与对照组等位基因F分布频率分别为42%(142/338)和51%(159/312),P>0.05,f分别为58%(196/338)和49%(153/312),P<0.05。结论:VDR基因FokⅠ多态性与BPH中组织学前列腺炎发生有一定关系。
Objective: To investigate the relationship between Fok Ⅰ polymorphism of vitamin D receptor (VDR) gene promoter and benign prostatic hyperplasia (BPH) and histopathological prostatitis. Methods: Polymorphisms of Fok Ⅰ in 79 patients with BPH complicated with prostatitis (inflammatory group) and 81 patients with pure BPH (control group) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) The frequency distribution of genotypes and alleles was examined and analyzed. Results: The distribution of genotypes in the inflammation group was FF (27/81), Ff: 30% (24/79), ff: 43% (34/79) : 36% (29/81), ff: 31% (25/81) respectively. There was a significant difference in the distribution of ff genotype between the two groups (P <0.05). The frequencies of allele F in inflammatory group and control group were 42% (142/338) and 51% (159/312) respectively, P> 0.05, f were 58% (196/338) and 49% (153/312 ), P <0.05. Conclusion: The Fok Ⅰ polymorphism of VDR gene is related to the occurrence of histological prostatitis in BPH.