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用2,4,5—T,pCPA,dicamba三种激素的0.5,1.0,1.5,2.0,2.5mg/l5种浓度分别培养小麦F_1和F_2代花药,均能获得花粉愈伤组织。其中,接种在2,4,5—T5种浓度培养基上的花粉愈伤组织平均诱导率分别为2.50％,3.62％,7.03％,7.12％,3.71％;接种在pCPA 5种浓度培养基上的花粉愈伤组织平均诱导率分别为7.00％,6.48％,5.54％,8.54％,8.18％;接种在dicamba 5种浓度培养基上的花粉愈伤组织平均诱导率分别为10.80％,16.49％,11.16％,8.91％,7.46％。当2,4,5-T的浓度为0.5—2.0mg/l,pCPA和dicamba的浓度分别为1.0,1.5和2.0mg/l时,均可使花粉愈伤组织一步成苗或成根,但一步成苗率偏低。
Pollen callus was obtained by culturing F_1 and F_2 anthers with concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 mg / l at concentrations of 2,4,5-T, pCPA and dicamba respectively. The average callus induction rate of pollen callus cultured in 2, 4, 5-T5 medium was 2.50%, 3.62%, 7.03%, 7.12% and 3.71%, respectively; inoculated on 5 concentrations of pCPA medium The average callus induction rates of pollen callus were 7.00%, 6.48%, 5.54%, 8.54% and 8.18%, respectively. The average callus induction rate of pollen callus on dicamba 5 medium was 10.80% and 16.49% 11.16%, 8.91%, 7.46%. Pollen callus could be made in one step or into roots when the concentration of 2,4,5-T was 0.5-2.0 mg / l and the concentrations of pCPA and dicamba were 1.0, 1.5 and 2.0 mg / l, respectively One step into the seedling rate is low.