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目的探讨直肠癌中VEGF-C及COX-2表达的关系及意义。方法用COX-2特异性抑制剂NS-398及其催化产物PGE2作用于大肠癌LOVO细胞,应用Western blot方法检测VEGF-C蛋白表达。应用免疫组化方法检测45例直肠腺癌组织VEGF-C及COX-2表达情况。结果 NS-398 明显抑制LOVO细胞VEGF-C蛋白的表达。经PGE2作用后,VEGF-C蛋白表达上调。直肠癌组织中 VEGF-C及COX-2的表达具有明显相关性。结论COX-2上调VEGF-C表达,促进肿瘤淋巴转移。
Objective To investigate the relationship between the expression of VEGF-C and COX-2 in rectal cancer and its significance. Methods Colorectal cancer LOVO cells were treated with COX-2 specific inhibitor NS-398 and its catalytic product PGE2. The protein expression of VEGF-C was detected by Western blot. Immunohistochemistry was used to detect the expression of VEGF-C and COX-2 in 45 cases of rectal adenocarcinoma. Results NS-398 significantly inhibited the expression of VEGF-C protein in LOVO cells. After PGE2 effect, VEGF-C protein expression was up-regulated. The expression of VEGF-C and COX-2 in rectal cancer tissues was significantly correlated. Conclusion COX-2 up-regulates the expression of VEGF-C and promotes tumor lymphatic metastasis.