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目的:比较液相色谱-串联质谱(LC-MS/MS)法与微粒子酶联免疫(MEIA)法测定人血浆中地高辛浓度的差异。方法:分别采用LC-MS/MS法与MEIA法对质控样品及50例患者血浆样品中的地高辛浓度进行检测,采用配对t检验比较分析两种方法的检测结果。结果:质控样品检测结果显示,两种方法检测结果间的差异无统计学意义(P>0.05);50例患者血浆样品检测结果显示,MEIA法检测结果明显高于LC-MS/MS法,两者差异有统计学意义(P<0.01)。结论:MEIA法检测结果与临床实际情况相比存在一定的差异,LC-MS/MS法更适于临床地高辛血药浓度的检测。
Objective: To compare the difference of digoxin concentrations in human plasma between liquid chromatography-tandem mass spectrometry (LC-MS / MS) and micro-particle enzyme immunoassay (MEIA). Methods: The plasma concentrations of digoxin in plasma samples and control samples from 50 patients were detected by LC-MS / MS and MEIA respectively. The paired t test was used to compare the results of two methods. Results: The results of quality control samples showed no significant difference between the two methods (P> 0.05). The results of plasma samples from 50 patients showed that the results of MEIA assay were significantly higher than that of LC-MS / MS, The difference was statistically significant (P <0.01). CONCLUSION: The results of MEIA assay are different from those of clinical practice. The LC-MS / MS method is more suitable for the detection of clinical digoxin concentration.