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目的:观察大鼠肺血栓栓塞症中血清、肺血管内皮细胞上P-选择素(P-selectin,Ps)的变化,并探讨血必净注射液对其的影响。方法:颈静脉注入自体血凝块制备动物模型。将105只健康雄性SD大鼠按随机数字表法分为对照组、栓塞组和血必净组。每组又分为术后1h、3h、1天、3天、7天5个时间段,每个时间段7只。留取肺组织和血清,观察各组肺组织病理变化,Western blot法检测肺血管内皮细胞上Ps的蛋白表达水平,ELISA法测血清中可溶性P-选择素(sP-selectin,sPs)含量。结果:肺栓塞后病理可见肺动脉中血栓栓塞,炎症反应明显。肺血管内皮上Ps的蛋白表达水平及血清中sPs含量在栓塞后1h开始显著增高(P<0.01)。血必净组经治疗后,病理损伤减轻,肺血管内皮上Ps的蛋白表达水平于1h后下降,与栓塞组比较,有显著性差异(P<0.01),血清sPs含量也下降,与栓塞组比较,1天后差异均有统计学意义(P<0.01或P<0.05)。结论:血必净注射液可能通过降低肺血栓栓塞时Ps表达,从而减轻炎症和凝血纤溶失衡对肺组织的损害,对肺组织具有一定的保护作用。
OBJECTIVE: To observe the changes of P-selectin (Ps) in serum and pulmonary vascular endothelial cells in rats with pulmonary thromboembolism and to investigate the effect of Xuebijing injection on them. METHODS: An animal model was prepared by injecting autologous blood clots into the jugular vein. 105 healthy male SD rats were randomly divided into control group, embolization group and Xuebijing group. Each group was divided into five time periods of 1h, 3h, 1 day, 3 days, and 7 days after surgery, with 7 in each period. Lung tissue and serum were collected and the pathological changes of lung tissues were observed. Western blot was used to detect Ps protein expression on pulmonary vascular endothelial cells. Serum ELISA was used to measure the content of soluble P-selectin (sPs) in serum. Results: Pulmonary arterial thromboembolism was observed after pulmonary embolism. The inflammatory reaction was obvious. The expression of Ps protein in the pulmonary vascular endothelium and serum sPs content increased significantly at 1 h after embolization (P<0.01). In the Xuebijing group, the pathological lesions were relieved, and the Ps protein expression level on the pulmonary vascular endothelium decreased after 1 h. Compared with the embolization group, there was a significant difference (P<0.01). The serum sPs content also decreased, and the embolization group For comparison, the difference was statistically significant one day later (P<0.01 or P<0.05). Conclusion: Xuebijing injection may reduce the damage of lung tissue caused by inflammation and coagulation and fibrinolysis imbalance by reducing the expression of Ps during pulmonary thromboembolism, and it has a protective effect on lung tissue.