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采用无血清培养体系 ,研究肿瘤细胞自分泌调控机制。将人肝癌细胞培养在无血清无外源分裂因子培养基中 ,采用细胞原位计数法和免疫组织化学染色法 ,观察不同时相人肝癌细胞增生率的变化以及碱性成纤维细胞生长因子 (bFGF)的表达情况。结果 :人肝癌细胞生长良好 ,其增生速度与其分泌的bFGF量成正比。提示 :肝癌细胞在无外源生长因子的条件下通过分泌bFGF ,以自分泌和旁分泌的方式作用于自身和相邻细胞 ,促进细胞生长和增生。
Using serum-free culture system to study the autocrine regulation mechanism of tumor cells. The human hepatocellular carcinoma cells were cultured in serum-free medium without exogenous cytokines. The cells were counted in situ and immunohistochemistry to observe the changes of the proliferation of human hepatocellular carcinoma cells in different phases and the changes of basic fibroblast growth factor bFGF) expression. Results: Human hepatocellular carcinoma cells grew well and their proliferation rate was proportional to the amount of bFGF they secreted. Hint: In the absence of exogenous growth factor, hepatoma cells secrete bFGF, which acts on itself and adjacent cells in an autocrine and paracrine manner to promote cell growth and proliferation.