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目的:观察清肠栓对急性溃疡性结肠炎(UC)大鼠结肠黏膜粒-巨噬细胞集落刺激因子(GM-CSF)和粒细胞集落刺激因子(G-CSF)的影响。方法:48只SD大鼠随机分为正常组、模型组、清肠栓组和柳氮磺胺吡啶(SASP)组,每组12只。用三硝基苯磺酸(TNBs)复制大鼠急性溃疡性结肠炎模型。造模后第3天开始给药,连续给药7d后处死大鼠。采用免疫组化、实时荧光定量PCR检测结肠组织中GM-CSF、G-CSF蛋白及mRNA表达。结果:模型组GM-CSF和G-CSF蛋白表达较正常组升高(P<0.05),清肠栓组及SASP组均较模型组降低(P<0.05);模型组GM-CSF和G-CSFmRNA表达较正常组明显增高(P<0.05),清肠栓组及SASP组均较模型组减低(P<0.05)。结论:清肠栓可通过调节结肠黏膜GM-CSF、G-CSF蛋白及基因表达,以治疗急性溃疡性结肠炎。
Objective: To observe the effects of Qingchang suppository on colon mucosal macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) in rats with acute ulcerative colitis (UC). Methods: Forty-eight SD rats were randomly divided into normal group, model group, Qingchang suppository group and sulfasalazine (SASP) group, with 12 rats in each group. Rat models of acute ulcerative colitis were replicated with trinitrobenzene sulfonic acid (TNBs). Administration was started on the third day after the model was established, and the rats were sacrificed 7 days after the continuous administration. Immunohistochemistry and real-time fluorescent quantitative PCR were used to detect the expression of GM-CSF and G-CSF protein and mRNA in colon tissue. Results: The expression of GM-CSF and G-CSF protein in the model group was higher than that in the normal group (P<0.05). The Qingchangshuan group and SASP group were lower than the model group (P<0.05); the model group GM-CSF and G- The expression of CSF mRNA was significantly higher than that of the normal group (P<0.05). Both Qingchangshuan and SASP groups were lower than the model group (P<0.05). Conclusion: Qingchang suppository can treat acute ulcerative colitis by regulating the expression of GM-CSF, G-CSF protein and gene in colonic mucosa.