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鼠单克隆抗体(MoAb130)能特异地与iC_3h、C_3dg和C_3d上存在的一种新抗原结合,而很少或完全不与C_3,C_3b或C_3c结合。本文作者用这种新抗体(MoAb130)发展了一种不采用PEG处理而直接定量测定iC_3d和C_3d新抗原的简单方法。MoAb130可从鼠腹水溶液纯化而来或通过蛋白质A的亲和色谱法从细胞培养上清液中得到。血浆样品从正常志愿者(NHP)
The murine monoclonal antibody (MoAb130) specifically binds to a novel antigen present on iC_3h, C3_g and C3_, with little or no binding to C3, C3b or C3_c. The authors used this new antibody (MoAb130) to develop a simple method for the direct quantitation of the new iC_3d and C_3d antigens without PEG treatment. MoAb130 can be purified from aqueous ascitic fluid or obtained from cell culture supernatants by protein A affinity chromatography. Plasma samples from normal volunteers (NHP)