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以余甘子(phyllanthusemblicaL.)下胚轴为外植体,通过器官发生途径诱导形成不定芽,探讨不同激素组合对下胚轴器官发生和植株再生的影响,以期建立有效的再生体系,为以后的遗传转化研究奠定基础。结果表明,在附加0.1μmol/LNAA、5μmol/LBA和30g/L蔗糖MS培养基上培养,5周后不定芽再生频率达87.5%,平均每个外植体再生芽数为9.7个,为最佳组合。将分化的不定芽转移至含有10μmol/LIBA的MS培养基上,5周后生根,发育成健康的植株,生根率在29.2%。
Using hypocotyls of phyllanthusemblica L. as explants, adventitious buds were induced through organogenesis pathways, and the effects of different hormone combinations on organogenesis and plant regeneration of hypocotyls were discussed in order to establish an effective regeneration system for future generations Genetic transformation research laid the foundation. The results showed that the frequency of adventitious buds regeneration reached 87.5% after 5 weeks of culture with MS medium supplemented with 0.1 μmol / L NAA, 5 μmol / L BA and 30 g / L sucrose, and the average number of regenerated shoots per explant was 9.7 Good combination. Differentiated adventitious buds were transferred to MS medium containing 10 μmol / L IBA and then rooted after 5 weeks to develop into healthy plants with a rooting rate of 29.2%.