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采用阳离子交换树脂分离提取核苷肽类农用抗生素新奥霉素。实验结果表明,在8种阳离子交换树脂中,7320树脂吸附效果最好,在pH=7时,树脂的交换容量最大;3%氨水可高效地将新奥霉素从7320树脂上解析下来,解析率为95.00%。静态吸附动力学符合Lagergren准二级速率方程,颗粒内扩散为影响吸附速率的主要控速步骤,吸附速率常数为k=0.00738g/(mg·min)。吸附等温线符合Langmuir方程,由热力学平衡方程计算得ΔH=60.93kJ/mol,ΔS>0,ΔG<0,表明该交换过程是自发的、吸热、熵增加的过程。在动态实验中,发酵液以1BV/h上样、3%氨水以2BV/h进行解吸,解吸液经减压浓缩得到褐色膏状物质,新奥霉素得率为79.96%。
Separation and Extraction of Nucleoside Peptide Agricultural Antibiotic Polymyxin with Cation Exchange Resin. The experimental results show that the adsorption capacity of 7320 resin is the best among the eight cation exchange resins, and the exchange capacity of resin is the highest at pH = 7. The 3% ammonia solution can effectively resolve the NPA from 7320 resin, The rate is 95.00%. The kinetics of static adsorption accords with the Lagergren quasi-second-order rate equation. The intraparticle diffusion is the main step of controlling the adsorption rate. The adsorption rate constant is k = 0.00738g / (mg · min). The adsorption isotherms fit the Langmuir equation and ΔH = 60.93kJ / mol, ΔS> 0 and ΔG <0, respectively, indicating that the exchange process is a spontaneous, endothermic and entropy-increasing process. In the dynamic experiment, the fermentation broth was loaded with 1BV / h and 3% ammonia water at 2BV / h. The desorption solution was concentrated under reduced pressure to obtain a brown paste, yield of which was 79.96%.